Tycho

The Tycho NT.6, from NanoTemper Technologies, trades flexibility for speed: it runs a single fixed thermal-ramp protocol with no method development required, and returns a result — an inflection temperature (Ti) and a fluorescence-brightness readout — from just 10 µL of sample in about three minutes. Rather than a full stability profile, it is built to answer a narrower, faster question: does this sample look like good-quality, folded protein or not.

Sample volume: 10 µL

Measurement time: about 3 minutes per sample

Protocol: single fixed thermal ramp, no method development

Output: inflection temperature(s) Ti, sample brightness/quality flags

Tycho is the platform's rapid quality-control check rather than a characterization tool — used to confirm a newly purified batch, a shipped sample, or a freshly thawed aliquot is still properly folded before committing it to a longer ITC, SPR or full nanoDSF/DSC experiment.