Biacore T200

The Biacore T200, from Cytiva, is the platform's SPR system: four flow cells connected in series (an experiment typically uses a reference and an active cell in pairs — 1&2 or 3&4), an integrated degasser, and an autosampler that accepts 96- or 384-well plates for unattended kinetic screens. Sensitivity reaches down to 1 resonance unit (RU), with baseline noise below 0.03 RU, which is what makes small-molecule analytes — far below the mass of a typical protein — detectable at all by SPR.

Flow cells: 4, connected in series

Sensitivity: detection down to 1 RU; baseline noise <0.03 RU

Flow rate range: 1–100 µL/min

Injection volume: 2–350 µL

Temperature range: 4–45 °C

Autosampler: 96- or 384-well plate compatible, up to 384 samples per run

The T200 is the platform's only SPR platform, used across the full range of SPR work described on the method page — kinetics, epitope mapping, and small-molecule target engagement — with the autosampler making it practical to run a full concentration series, or a large epitope-mapping matrix, as a single unattended queue.