ITC200

The MicroCal iTC200, from Malvern Panalytical, is the smaller-volume, semi-automated member of PINT's ITC line. Its 200 µL sample cell and roughly 280 µL sample requirement make it the platform's choice when material is genuinely scarce — as little as 10 µg of protein is enough to run a titration. Injections as small as 0.1 µL are possible, with better than 1% precision at a 1.5 µL injection volume, and the Hastelloy sample and reference cells resist the buffers and biological samples routinely used at the platform. Operation is semi-automated: an operator loads the cell and syringe by hand for each run, and a titration typically takes under an hour, with 8 to 12 experiments realistically completed in an 8-hour day.

Sample cell volume: 200 µL

Sample volume per experiment: ~280 µL

Injection volume: as low as 0.1 µL, <1% variability at 1.5 µL

Sensitivity: measurable with as little as 10 µg of protein

Affinity range: KD 10 nM–10 mM directly; down to the low picomolar range via competitive ITC

Cell material: Hastelloy alloy

Operation: semi-automated, manual cell/syringe loading

At PINT, the iTC200 is the workhorse for exploratory and lower-throughput ITC work — early-stage binding checks, small compound sets, or any project where sample quantity is the limiting factor rather than the number of conditions to test. For higher-throughput or unattended runs across many titrations, the platform's automated PEAQ-ITC is the better fit; the two instruments share the same 200 µL cell chemistry and affinity range, so results are directly comparable between them.